is necessary for successful wasp parasitization. Bracoviruses (PDV), is a group deriving from Nudiviruses and are baculovirus-related type of viruses.
Innate immune responses caused by an endoparasitoid insect virus. To study the mechanisms of immunosuppression in lepidopteran insects following parasitization by hymenopteran endoparasitoids, we have expressed CcBV proteins and investigated their interactions with host’s (Manduca sexta) haemocyte proteins. In fact, CcV1 protein has been shown to interfere with host cellular and humoral immune responses. (1,2,3,4)
Differential inhibition of the Imd and Toll pathways in lepidopteran cells by CcBV ankyrin-repeat proteins. One of the largest bracovirus (CcBV) protein families is the ankyrin-repeat protein family which is homologous to mammalian IκBa. This family includes nine members Ank1-9 and is thought to interfere with the host’s induced innate immune responses. Introducing transcriptional assays we found that CcBV Anks exhibit differential inhibition on the Bombyx mori, Rel/NFκB (Relish1-d2 and RelB), transcriptional activities. Subcellular localization of Anks suggests functional and spatial differences between the various members of this family (5).
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Lapointe R, Wilson R, Vilaplana L, O'Reilly DR, Falabella P, Douris V, Bernier-Cardou M, Pennacchio F, Iatrou K, Malva C, Olszewski JA. Expression of a Toxoneuron nigriceps polydnavirus-encoded protein causes apoptosis-like programmed cell death in lepidopteran insect cells. J Gen Virol. 2005, 86:963-71.
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Espagne E, Douris V, Lalmanach G, Provost B, Cattolico L, Lesobre J, Kurata S, Iatrou K, Drezen JM, Huguet E. A virus essential for insect host-parasite interactions encodes cystatins. J Virol. 2005, 79: 9765-76.
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Douris V, Swevers, L, Labropoulou V, Andronopoulou E, Georgoussi Z. and Iatrou K. Stably transformed insect cell lines: tools for expression of secreted and membrane-anchored proteins and high throughput screening platforms for drug and insecticide discovery. Adv. in Virus Res., 2006, 68: 113-156.
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Labropoulou V, Douris V, Stefanou D, Magrioti C, Swevers L, and Iatrou K. Endoparasitoid wasp bracovirus-mediated inhibition of hemolin function and lepidopteran host immunosuppression. Cell. Microbiol. 2008, 10: 2118-28.
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Magkrioti C, Iatrou K. and Labropoulou V. Differential inhibition of BmRelish1-dependent transcription in lepidopteran cells by bracovirus ankyrin-repeat proteins. Insect Biochem Mol Biol 2011, 12: 993-1002.
