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NCSR Demokritos

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Light Microscopy Unit

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The Light Microscopy Unit of the Institute of Biosciences and Applications of NCSR "Demokritos" has been operating since July 2016 with a new generation, modern equipment, serving the imaging needs of both the research laboratories of the Institute and other Institutes of NCSR "D", as well as external Research centers and Universities


  • A Leica TCS SP8 MP multiphoton confocal microscope with Spectra Physics Mai Tai infrared laser source and fully motorized (3-axis motorized stage) microscope stage. The system is accompanied by chamber that enable tight control of virtually all environmental variables(humidity, temperature, CO2, O2, N2)
  • Α Nikon E600 upright optical microscope


Available services include a wide range of application of optical microscopy:

  • Multi-Channel Fluorescence Microscopy, covering UV, Visible and Infrared Spectrum
  • Multi-color 3D Imaging
  • Live Cell Imaging
  • 2-Photon Microscopy
  • Second Harmonic imaging
  • Förster Resonance Energy Transfer (FRET) to monitor molecular interactions in live or fixed specimens
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Spectral unmixing of fluorescent dyes
  • Cell/tissue Colocalization analysis
  • Calcium Imaging
  • Differential Interference Contrast (DIC) microscopy (known as Nomarski microscopy)
  • Image processing and analysis (with specialized software programss uch as ImageJ / Fiji and Imaris (Bitplane)


  • Dimitris Kletsas, Research Director, Director of the Institute of Biosciences and Applications, Administrative and Scientific Responsible
  • Vassiliki Labropoulou, Senior Researcher
  • Haris Pratsinis, Senior Researcher
  • Marina Sagnou, Associate Researcher
  • Alexandros Athanasopoulos, Research Assistant


Dimitris Kletsas

+30210 6503583

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Alexandros Athanasopoulos

+30210 6503534

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