Circular Dichroism Laboratory

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CIRCULAR DICHROISM (CD) LABORATORY

Address

Institute of Biosciences & Applications

NCSR "Demokritos" , Agia Paraskevi, Athens 153 10

Contact details

Phone: +30.210.6503558

Fax: +30.210.6511767

e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

Personnel

Dr. Metaxia Vlassi, Research Director

Dr. Maria Pelecanou, Research Director

Dr. Angeliki Panagiotopoulou, Technical Specialist

Users' Committee:

Dr. Metaxia Vlassi (IB-A)

Dr. Maria Pelecanou (IB-A)

Dr. Angeliki Chroni (IB-A)

Dr. Georgios Nounesis (INRASTES)

Dr. Stratos Stratikos (INRASTES)

 

Description of the laboratory

Circular Dichroism (CD) is a well-established spectroscopic technique based on the differential absorption of circularly polarized light from optically active molecules and it is widely used for the study of conformation and interactions of biological macromolecules, like peptides, proteins and nucleic acids. Advantages of CD include its high sensitivity - requiring the use of small amount of sample - and its versatility in the fine tuning of experimental conditions

 

The equipment of the CD laboratory includes a JASCO-715 spectropolarimeter equipped with Peltier system for temperature control. This CD infrastructure was acquired in 1998 within the framework of the “Center for Crystallographic Studies of Macromolecules (CCM)” which was financed through a grant (EPET) from the General Secretariat for Research and Technology as a network of three Institutes of NCSR “D” (the former: Biology, Physical Chemistry, Radioisotopes & Radiodiagnostic Products) and other Greek academic institutions.

 

The CD lab is located at Room Y35 of IB-A, has been operating since 1998 under the supervision of research scientists of IB-A addressing mainly research projects of the academic community and is interconnected with other laboratories of NCSR "Demokritos" related to structural studies (NMR Laboratory, X-Ray Laboratory).

 

Activities/Services

The objective of the CD lab is to perform CD experiments on liquid samples. The CD method is highly accurate, sensitive, reproducible, and non-destructive to the sample. CD is ideal for: