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NCSR Demokritos

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Εργαστηριακό Σεμινάριο για καθηγητές Βιολογίας Δευτεροβάθμιας Εκπαίδευσης- Προγραμμα

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Laboratory workshop for secondary school
teachers
(11/9/2020 13/9/2020)
Friday 17:00-17:30 Registration 17:30-17:45 Welcome - Presentation of the NCSR Demokritos, Dr. George Nounesis, Director and Chairman of the Board of the NCSR Demokritos
17:45-18:15 Presentation of the Institute of Biosciences and Applications , presentation of the course, Dr. Dimitris Kletsas, Director of the Institute of Biosciences and Applications, NCSR Demokritos
18:15-18:30 Presentation of EMBL and ELLS Dr. Nikolaos Papadopoulos, EMBL
18:30-19:00 "Advanced techniques in molecular biology: Introduction to single-cell RNA sequencing" Dr. Nikolaos Papadopoulos, EMBL
19:00-19:45 “The cellular response to oxygen levels: Regulation of the hypoxia-inducible transcription factors HIF & therapeutic approaches” Prof. George Simos, School of Medicine, University of Thessaly
Saturday 10:00-14:00 Groups 1-3: Preparation of SDS-PAGE gels, preparation of protein samples, samples loading and running, staining and destaining of gels for protein bands visualization, Western blot analysis following SDS-PAGE Groups 4-6: Preparation of samples for PCR, PCR running, preparation of agarose gels, DNA samples running, DNA bands visualization 14:00-15:00 Lunch break 15:00-19:00 Groups 1-3: Preparation of samples for PCR, PCR running, preparation of agarose gels, DNA samples running, DNA bands visualization
Groups 4-6: Preparation of SDS-PAGE gels, preparation of protein samples, samples loading and running, staining and destaining of gels for protein bands visualization, Western blot analysis following SDS-PAGE
Instructors: Drs. I. Georgoussi, C. Gournas, G. Drossopoulou, V. Labropoulou, E. Mavrogonatou, H. Pratsinis, A. Prombona, V. Sophianopoulou, L. Swevers, A. Tzinia, G. Voutsinas.
Sunday 10:00-12:00 Theoretical principles of laboratory methods. Discussion of the results of experimental analyses performed on day 2. All Instructors 12:00-13:00 Real time-PCR and its application for the detection of viral infections Dr. Eleni Mavrogonatou, Researcher, Institute of Biosciences and Applications, NCSR Demokritos 13:00-14:00 Lunch break 14:00-14:30 Contacting literature search in biosciences (Peer-reviewed literature vs “grey” literature, PUBMED, Google Scholar databases). Information on NCBI databases. Dr. Angeliki Chroni, Research Director, Institute of Biosciences and Applications, NCSR Demokritos
14:30-17:00 Confocal microscopy (principles of optical and confocal microscopy, visit of confocal microscopy facility) Dr. Alexandros Athanassopoulos, Institute of Biosciences and Applications, NCSR Demokritos
17:00-17:30 Open discussion, Farewell

Laboratory workshop for secondary schoolteachers(11/9/2020 13/9/2020)

Friday

17:00-17:30 Registration

17:30-17:45 Welcome - Presentation of the NCSR Demokritos, Dr. George Nounesis, Director and Chairman of the Board of the NCSR Demokritos

17:45-18:15 Presentation of the Institute of Biosciences and Applications , presentation of the course, Dr. Dimitris Kletsas, Director of the Institute of Biosciences and Applications, NCSR Demokritos

18:15-18:30 Presentation of EMBL and ELLS Dr. Nikolaos Papadopoulos, EMBL

18:30-19:00 "Advanced techniques in molecular biology: Introduction to single-cell RNA sequencing" Dr. Nikolaos Papadopoulos, EMBL

19:00-19:45 “The cellular response to oxygen levels: Regulation of the hypoxia-inducible transcription factors HIF & therapeutic approaches” Prof. George Simos, School of Medicine, University of Thessaly

Saturday

10:00-14:00 Groups 1-3: Preparation of SDS-PAGE gels, preparation of protein samples, samples loading and running, staining and destaining of gels for protein bands visualization, Western blot analysis following SDS-PAGE Groups 4-6: Preparation of samples for PCR, PCR running, preparation of agarose gels, DNA samples running, DNA bands visualization

14:00-15:00 Lunch break

15:00-19:00 Groups 1-3: Preparation of samples for PCR, PCR running, preparation of agarose gels, DNA samples running, DNA bands visualizationGroups 4-6: Preparation of SDS-PAGE gels, preparation of protein samples, samples loading and running, staining and destaining of gels for protein bands visualization, Western blot analysis following SDS-PAGEInstructors: Drs. I. Georgoussi, C. Gournas, G. Drossopoulou, V. Labropoulou, E. Mavrogonatou, H. Pratsinis, A. Prombona, V. Sophianopoulou, L. Swevers, A. Tzinia, G. Voutsinas.

Sunday

10:00-12:00 Theoretical principles of laboratory methods. Discussion of the results of experimental analyses performed on day 2. All Instructors

12:00-13:00 Real time-PCR and its application for the detection of viral infections Dr. Eleni Mavrogonatou, Researcher, Institute of Biosciences and Applications, NCSR Demokritos

13:00-14:00 Lunch break

14:00-14:30 Contacting literature search in biosciences (Peer-reviewed literature vs “grey” literature, PUBMED, Google Scholar databases). Information on NCBI databases. Dr. Angeliki Chroni, Research Director, Institute of Biosciences and Applications, NCSR Demokritos

14:30-17:00 Confocal microscopy (principles of optical and confocal microscopy, visit of confocal microscopy facility) Dr. Alexandros Athanassopoulos, Institute of Biosciences and Applications, NCSR Demokritos

17:00-17:30 Open discussion, Farewell