The Eighth International Workshop on MOLECULAR BIOLOGY AND GENETICS OF THE LEPIDOPTERA |
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August 23-29, 2009 Orthodox Academy of Crete Workshop Programm |
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* Lunchbag and departure at 13:15; dinner at the excursion site. |
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Session Topics - Coordinators and Oral Presentations | ||
Session 1: | Whole Genome Sequencing, EST and Linkage Mapping Projects (D. Heckel, K. Mita, J. Marcus) |
10 talks |
Session 2: | Comparative Genomics, Evolution and Phylogeny (K. Gordon, M. Goldsmith, F. Sperling) |
6 talks |
Session 3: | Gene families and transcriptomics (K. Hiruma, L. Swevers) |
5 talks |
Session 4: | Development and Differentiation (R. Lecanidou, J. Nagaraju, F. Marec) |
10 talks |
Session 5: | Endocrinology and hormonal control of development |
9 talks |
Session 6: | Biochemistry – protein structure-function relationships (S. Hamodrakas, R. Hill) |
5 talks |
Session 7: | Immunity (M. Yamakawa, S. Herrero, O. Terenius) |
7 talks |
Session 8: | Neurobiology and behavior (R. Vogt, H. Vogel) |
5 talks |
Session 9: | Functional Genomics and post-genomic tools and applications (M. Bergoin, K. Iatrou) |
6 talks |
Detailed Workshop Program | ||
Sunday, August 23, 2009 | ||
12:00-18:00 | Registration and room assignment | |
18:00-20:30 | Welcome reception and dinner |
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Monday August 24, 2009 | ||
07:00 | Breakfast | |
08:15 | Introductory remarks by organizers and welcome by Dr. Konstantinos S. KENANIDIS, Director General of the OAC |
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08:30-13:00 | Session 1: Whole genome sequencing, EST and linkage mapping projects (Session Coordinators: D. Heckel, K. Mita, J. Marcus) |
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08:30 | 1.1 The genome of a lepidopteran model insect, the silkworm Bombyx mori Q. Xia and Kazuei MITA |
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09:00 | 1.2 Sequencing the genome of the major pest, the cotton bollworm, Helicoverpa armigera: a model for the evolution of complex ecological traits Karl GORDON, J. Oakeshott, P. Batterham, S. Richards, R. Gibb, J. Miller, T. Tay, P. East, O. Edwards, R. Good, C. Robin, D. Heckel, H. Vogel, R. Feyereisen, E. D’Alencon, H. Sezutsu, F. Legeai, H. Quesneville, C. Gagneur, S. Gimenez, A.-N. Volkoff, P. Fournier, A. Brun-Barale, S. Suraporn, M. Shimomura, K. Mita, S. Scherer, C. Qu, K. Worley |
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09:30 | 1.3 Synteny conservation and genome rearrangements between the silkworm Bombyx mori and the noctuid pests Helicoverpa armigera and Spodoptera frugiperda Emmanuelle d’ALENÇON, H. Sezutsu, F. Legeai, E. Permal, S. Bernard-Samain, S. Gimenez, C. Gagneur, F. Cousserans, M. Shimomura, A. Brun-Barale, T. Flûtre, A. Couloux, K. Gordon, K. Mita, H. Quesneville, P. Fournier and R. Feyereisen |
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10:00 | 1.4 Lepido-DB, a new bioinformatic resource for the annotation and cross-comparisons of lepidopteran genomes Fabrice LEGEAI, E. d'Alençon, F. Cousserans, H. Sezutsu, E. Permal, K. Gordon, H. Quesneville, R. Feyereisen and P. Fournier |
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10:30-11:00 | Coffee break | |
11:00 | 1.5 Syntheny and Chromosome Evolution in the Lepidoptera. Interspecies Cross Mapping between Papilio polytes and P. alphenor M. Obara, M. Furukawa, T. Murayama and Hiroshi HORI |
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11:30 | 1.6 Relative utility of DNA markers for delimiting Lepidoptera species, with particular reference to spruce budworms (Choristoneura fumiferana complex) Felix A.H. SPERLING, L.M. Lumley |
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12:00 | 1.7 The feature of Cecropia-ITR-MLE in BmTNML locus of B. mandarina distribute in Japan, China, Korea, and Taiwan Y. Kawanishi, Y. Kamauchi, Y. Banno, S.K. Nho, Y-F. Hsu, K. Ueda, M. Yago, K. Odagiri, H. Maekawa, Yumiko NAKAJIMA |
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12:30 | 1.8 Tracking Sex Chromosome Evolution In Lepidoptera Frantisek MAREC, M. Vitkova, M. Dalikova, J. Sichova, M. Sykorova, P. Nguyen, K. Sahara, W. Traut |
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13:00-13:15 | Minute-long poster previews – Posters #1-14 | |
13:30 | Lunch |
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16:30-18:00 | Poster Session 1 (with continuous coffee and tidbits) | |
18:00-19:00 | Session 1 (cont): Whole genome sequencing, EST and linkage mapping projects | |
18:00 | 1.9 Linkage map of the peppered moth, Biston betularia (Geometridae), including localization of the carbonaria morph switch and melanisation pathway genes A. van’t Hof, N. Edmonds, F. Marec & Ilik SACCHERI |
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18:30 | 1.10 Establishment of the segregation models for Bt toxins and the linkage analysis of resistant genes in the silkworm, Bombyx mori Osamu NINAGI, T. Takiguchi, M. Awaji, T. Yokoyama, T. Shimada, K. Yamamoto, and K. Miyamoto |
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19:00-20:45 | Session 2: Comparative genomics, evolution and phylogeny |
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19:00 | 2.1 Genes underlying aposematic colour patterns in Heliconius butterflies Simon W. BAXTER, C. Salazar, L. Maroja and C.D. Jiggins |
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19:30-19:45 | Break | |
19:45 | 2.2 Molecular evolution of a mimicry supergene by chromosomal rearrangements in Heliconius butterflies Matthieu JORON, L. Ferguson, N. Chamberlain, C. Jiggins, R. H. ffrench-Constant |
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20:15 | 2.3 Phylogenomic approaches to color pattern evolution and development Jeffrey M. MARCUS |
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21:00 | Dinner | |
Tuesday August 25, 2009 | ||
07:00 | Breakfast | |
08:30-10:00 | Session 2 (cont.): Comparative genomics, evolution and phylogeny | |
08:30 | 2.4 Comparative sequence signatures, host plant shifts and birth-death dynamics of a coevolutionary key innovation in a plant-insect interaction Heiko VOGEL, H.M. Heidel-Fischer, D.G. Heckel, and C.W. Wheat |
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09:00 | 2.5 Enzymatic adaptation of mulberry specialists to toxic sugar-mimic alkaloids in mulberry latex Takaaki DAIMON, Y. Meng, T. Yoshinaga, S. Katsuma, and T. Shimada |
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09:30 | 2.6 Genome-wide Annotation and Evolution of Insect Neuropeptide GPCRs and Their Ligands Cornelis J. P. GRIMMELIKHUIJZEN, E. Stafflinger, K.K. Hansen, M. Schneider, G. Cazzamali, M. Williamson, F. Hauser. |
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10:00-13:00 | Session 3: Gene families and transcriptomics |
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10:00 | 3.1 Manduca midgut transcriptomics Yannick PAUCHET, A. Papanicolaou, H. Vogel, D.G. Heckel, R.H. ffrench-Constant |
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10:30 | 3.2 Gastric and pancreatic lipases in the larval midgut of Epiphyas postvittana (Tortricidae): their roles in lipid digestion based on phylogenetic and gene expression studies. John T. CHRISTELLER, N.P. Markwick, J. Poulton |
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11:00-11:30 | Coffee break and group picture taking | |
11:30 | 3.3 Analysis of gene expression patterns in Lymantria spp. larvae feeding on different food sources – a cDNA-AFLP approach Kirsten EVERTZ, H. Vogel, D.G. Heckel, A. Reineke |
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12:00 | 3.4 The Caspase gene family Juliette COURTIADE, Y. Pauchet, H. Vogel and D.G. Heckel |
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12:30 | 3.5 Development of a microarray platform for studying ecdysone-controlled gene expression in the spruce budworm (Choristoneura fumiferana) Daniel DOUCET, D. Zhang, S. Bowman, T. Ladd and P.J. Krell |
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13:00-13:15 | Minute-long poster previews – Posters #15-28 | |
13:30 | Lunch | |
16:30-18:00 | Poster Session 2 (with continuous coffee and tidbits)
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18:00-21:00 | Session 4: Development and Differentiation (Session Coordinators: R. Lecanidou, J. Nagaraju, F. Marec) |
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18:00 | 4.1 Lifespan differences among the strains of the silkworm Satoshi MURAKAMI, T. Iwamatsu, Y. Kitazawa, M. Katada, W. Hara, H. Kojima, K. Takahashi, T. Yokoyama, O. Ninagi |
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18:30 | 4.2 Transcriptional activity of the W chromosome Walther TRAUT, F. Marec and K. Sahara |
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19:00 | 4.3 Two functional Doublesex proteins are produced in the female silkmoths and are essential for sexual differentiation J. N. Shukla and Javaregowda NAGARAJU |
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20:00 | 4.4 wnt1 plays a crucial role in the spot marking formation on larval segment of Lepidoptera Junichi YAMAGUCHI, K. Yamamoto, J. Narukawa, Y. Banno, K. Mita, and H. Fujiwara |
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20:30 | 4.5 Proneural genes and the regulation of olfactory sensilla phenotypes Richard VOGT and R. Campbell |
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21:15 | Dinner | |
Wednesday August 26, 2009 |
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07:00 | Breakfast | |
08:30-11:00 | Session 4 (cont.): Development and Differentiation | |
08:30 | 4.6 Cloning and Characterization of a Gasp Homolog From the Spruce Budworm, Choristoneura fumiferana, and Its Putative Role in Cuticle Formation A. Nisole, D. Stewart, S. Bowman, D. Zhang, D. Doucet, Michel CUSSON |
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09:00 |
4.7 The three musketeers and the control of Bombyx mori chorion gene expression A. Papantonis, S. Tsatsarounos and R. LECANIDOU |
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09:30 | 4.8 Expression of Hsp genes in response to diapause and thermal stress in Sesamia nonagrioides (Lepidoptera: Noctuidae) Anna Kourti, T. Gkouvitsas and D. KONTOGIANNATOS |
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10:00 | 4.9 Carotenoid Cocoon Coloration is Controlled by a Combination of a CD36/SR-BI-related Transmembrane Receptor and a Cytosolic START Lipid-binding Protein in the Silkworm Bombyx mori Takashi SAKUDOH, T. Iizuka, J. Narukawa, H. Sezutsu, S. Kuwazaki, Y. Banno, N. Takada, H. Fujimoto, K. Kadono-Okuda, K. Mita, T. Tamura, K. Yamamoto, K. Tsuchida |
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10:30 | 4.10 Acetylcholinesterase genes in the Glanville fritillary butterfly (Melitaea cinxia; Lepidoptera: Nymphalidae). Shiqi LUO, C. Shu, R.J. Wang, C.R. Xu |
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11:00-11:30 | Coffee break | |
11:30-13:00 | Session 5: Endocrinology and hormonal control of development (Session Coordinators: R. Hormann, R. Palli, G. Smagghe) |
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11:30 | 5.1 Relationship between Halloween gene expression and ecdysteroids biosynthesis in cotton leafworm, Spodoptera littoralis Masatoshi IGA and G. Smagghe |
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12:00 | 5.2 The potential roles of the Receptor for Activated C Kinase 1 in spruce budworm, Choristoneura fumiferana Guoxing QUAN, D. Doucet, T. ladd, P.J. Krell and B.M. Arif |
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12:30 | 5.3 Regulation of juvenile hormone biosynthesis by 20-hydroxyecdysone and peptide hormones Kiyoshi HIRUMA, Y. Kaneko, T. Kinjoh, and M. Kiuchi |
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13:30 | Lunch box and bus boarding for excursion (dinner on site) |
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21:00 | Return to the Academy | |
Thursday August 27, 2009 |
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07:00 | Breakfast | |
08:30-12:00 | Session 5 (cont.): Endocrinology and hormonal control of development
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08:30 | 5.4 Differential action of juvenile hormone analog methoprene in moths and mosquitoes Subba R. PALLI, R. Parthasarathy and H. Bai |
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09:00 | 5.5 Molecular characterization of juvenile hormone network genes in the silkmoth, Bombyx mori Tetsuro SHINODA, T. Kayukawa, C. Minakuchi#, T. Togawa, Y. Shimizu, M. Yoshiyama, M. Murata, I. Kobayashi, T. Tamura, and K. Mita |
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09:30 | 5.6 Effects of juvenile hormone analogue, fenoxycarb, on remodeling of Bombyx mori midgut during larval-pupal metamorphosis Ebru GONCU, O. Parlak |
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10:00 | 5.7 Characterization and Functional Analysis of Juvenile Hormone Esterase-Related Genes in Silkworm, Bombyx mori Takuya TSUBOTA, M. Shimomura, T. Ogura, A. Seino, T. Nakakura, K. Mita, T. Shinoda, T. Shiotsuki |
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10:30-11:00 | Coffee break | |
11:00 | 5.8 RNA interference as a tool to analyze effects of allatotropins and allatostatins in the fall armyworm, Spodoptera frugiperda Klaus H. HOFFMANN, M. Griebler, M. Meyering-Vos |
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11:30 | 5.9 Physiological consequences of ETH-receptor expression in Bombyx mori Dušan ŽITŇAN, I. Daubnerová, L. Roller and M.E. Adams |
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12:00-13:00 |
Session 6: Biochemistry – protein structure-function relationships (Session Coordinators: S. Hamodrakas, R. Hill) |
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12:00 | 6.1 The silkworm mutant lemon (lemon lethal) is a potential insect model for human Sepiapterin reductase deficiency Yan MENG, S. Katsuma, T. Daimon, Y. Banno, K. Uchino, H. Sezutsu, T. Tamura, K. Mita, and T. Shimada |
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12:30 | 6.2 Recent Insights into the Structure of the Mamestra configurata Type I Peritrophic Matrix Dwayne D. HEGEDUS, M.A. Erlandson, U. Toprak, C. Gillott and S. Karcz |
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13:15 | Lunch | |
16:30-18:00 | Outbreak Session on “Lepidoptera and miRNAs” (coordinated by K. Gordon) (with continuous coffee and tidbits) |
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18:00-19:30 |
Session 6 (cont.): Biochemistry – protein structure-function relationships | |
18:00 | 6.3 The PK/PBAN family of insect neuropeptides: progress toward the design of novel insect control agents M. ALTSTEIN, M. Davidovitch, O. Ben-Aziz and A. Hariton |
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18:30 | 6.4 Ecdysone Receptor Function and Structure Graham LD, Hannan GN, Pawlak A, Noyce L, Tohidi-Esfahani D, Pollard M, Howell L, Fernley R, Lovrecz G, Lu L, Phan T, Carmichael J, Pilling P, Johnson WM, Bliese M, Turner K, Streltsov V, Peat T, O’Meara T, Winkler D, Lawrence MC and Ronald J HILL |
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19:00 | 6.5 Silkmoth Chorion Proteins: A Protective Role For Amyloid V.A. Iconomidou and Stavros J. HAMODRAKAS |
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19:30-19:45 | Break | |
19:45-21:15 | Session 7: Immunity (Session Coordinators: M. Yamakawa, S. Herrero, O. Terenius) |
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19:45 | 7.1 Spodoptera frugiperda X-Tox protein, an immune related defensin rosary, has lost the function of ancestral defensins D. Destoumieux-Garzón, M. Brehelin, P. Bulet, Y. Boublik, P.-A. Girard, S. Baghdiguian, R. Zumbihl, Jean-Michel ESCOUBAS |
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20:15 | 7.2 Recent studies on immune-related hemolymph protein Scolexin, described as a serine proteinase homolog, in the tobacco hornworm, Manduca sexta Fırat GÖKÇEN, Tina Trenczek |
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20:45 | 7.3 The role of Ankyrin-repeat proteins of the polydnavirus CcBV on lepidopteran immunity signaling pathways. Christiana MAGKRIOTI, K. Iatrou and V. Labropoulou |
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21:30 | Dinner | |
Friday August 28, 2009 |
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07:00 | Breakfast | |
08:30-10:30 | Session 7 (cont.): Immunity | |
08:30 | 7.4 Endocytotic capabilities of Manduca sexta hemocytes Anke MUELLER, T. Trenczek |
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09:00 | 7.5 Superfamily-dependency of Hemolin involvement in viral defence Olle TERENIUS, H.J.R. Popham, M.H. Beck, M. Strand and K.S. Shelby |
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09:30 | 7.6 Danger signals in lepidopteran innate immunity Andreas VILCINSKAS and B. Altincicek |
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10:00 | 7.7 Dietary-dependent trans-generational priming of immunity in an insect herbivore, Trichoplusia ni Dalial FREITAK, D.G. Heckel, H. Vogel, A. Vilcinskas |
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10:30-11:00 | Coffee break
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11:00-13:00 | Session 8: Neurobiology and behavior (Session Coordinators: R. Vogt, H. Vogel) |
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11:00 | 8.1 Olfactory receptors of the tobacco hornworm, Manduca sexta Ewald GROSSE-WILDE, R. Stieber, B.S. Hannson and D. Wicher |
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11:30 | 8.2 An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research S. Malpel, F. Legeai, F. Cousseran, C. Merlin, M.-C. François, C. Monsempes, M. Maïbèche-Coisné, F. Gavory, P. Wincker, Emmanuelle JACQUIN-JOLY |
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12:00 | 8.3 Molecular elements of pheromone reception in moths Jürgen KRIEGER, M. Forstner, E. Grosse-Wilde, T. Gohl, E. Bouché, I. Gondesen, H. Breer |
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12:30 | 8.4 Genetic analysis of the sex pheromone communication system in Ostrinia nubilalis A. Groot, S. Olssen, Z. Karpati, T. Dekker, J.-M. Lassance, C. Löfstedt, B. Hansson, and David G. HECKEL |
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13:00 | 8.5 Host plant products enhance the responses of the codling moth and other fruit crop tortricids to their sex pheromone Patrick M. GUERIN, D. Schmera, D. Schmidt-Büsser, M. von Arx and P. G. Becher |
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13:45 | Lunch | |
16:30-17:30 | Outbreak Session on "Lep genome annotation and bioinformatics solutions" (coordinated by M. Goldsmith, Alexie Papanicolaou and Fabrice Legeai) (with continuous coffee and tidbits) |
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17:30-20:30 | Session 9: Functional Genomics - post-genomic tools and applications (Session Coordinators: M. Bergoin, K. Iatrou) |
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17:30 | 9.1 Construction of enhancer trap system and Bombyx Trap DataBase for the analysis of gene function in silkworm Bombyx mori Hideki SEZUTSU, K. Uchino, T. Daimon, J. Sato, M. Shimomura, H. Minami, Y. Miyashita, C. Sato, I. Kobayashi, K.-I. Tatematsu, T. Iizuka, N. Yonemura, T. Shimada, K. Mita, T. Tamura |
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18:00 | 9.2 Construction of a binary gene expression system for the production of recombinant protein in the middle silk gland of the silkworm Bombyx mori Ken-ichiro TATEMATSU, I. Kobayashi, K. Uchino, H. Sezutsu, T. Iizuka, N. Yonemura, T. Tamura |
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18:30 | 9.3 Baculovirus engineering for development of safer gene therapy vectors and improved protein expression in mammalian cells Luc SWEVERS, R. Efrose, K. Iatrou |
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18:30-19:00 | Break | |
19:00 | 9.4 Baculoviruses associated with Mamestra configurata (Bertha Armyworm): Role of viral enhancing factor (VEF) in the infection process |
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19:30 | 9.5 Improving Baculovirus by Host Gene Manipulation A. Jakubowska, S. Caccia, K. Gordon, J. Ferre, Salvador HERRERO |
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20:00 | 9.6 Isolation and unique post-translational modification of a novel antiviral protein against nucleopolyhedrovirus in the silkworm, Bombyx mori |
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21:30 | Dinner and Cretan Night – Farewell Reception | |
Saturday August 29, 2009 | ||
08:00 | Breakfast | |
09:30-11:00 | Closing remarks and planning of the next Workshop (K. Iatrou and K. Gordon) | |
12:00 | Lunch and Departures | |
#1 |
On The Z-chromosome Synteny In Lepidoptera. |
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#2 |
BAC Clones As A Tool For The Study Of The Codling Moth Sex Chromosomes. |
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#3 |
Mapping resistance to Bt toxin Cry1Ab in Bombyx mori |
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#4 |
Laser Microdissection Of Codling Moth Z Chromosome For Zoo-FISH And Sequence Library. |
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#5 |
Isolation Of Novel Repetitive Sequences In Two Moths and a Butterfly By Means of PCR with TRAS Primers. |
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#6 |
Pheromone variability and evolution in the butterfly genus Bicyclus, and implication in its diversification |
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#7 |
Evaluation of the specificity and efficiency of ecdysone agonists in Bombyx mori and Spodoptera littoralis by in vitro reporter assays and in vivo toxicity assays. |
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#8 |
Molecular genetics of host adaptation and ecological speciation |
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#9 |
Receptors and transcription factors controlling secretory activity of Inka cells. |
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#10 |
Neurogenesis during post-embryonal development of the cotton leaf worm, Spodoptera littoralis. |
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#11 |
Localization of PER protein during spermatogenesis and development of the male reproductive system of the cotton leafworm (Spodoptera littoralis). |
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#12 |
Implication of the clock-gene period in the rhythm of H+-ATPase subunits expression in vas deferens of the cotton leaf worm, Spodoptera littoralis. |
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#13 |
Developmental and hormonal regulation of Krüppel homolog 1 in the silkworm, Bombyx mori. |
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#14 |
Morphological evolution of antennal structure in Lepidoptera. |
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#15 |
Toward the functional characterization of mosquito smell components in lepidopteran tissue culture cells. |
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#16 |
Baculovirus engineering: A viral RNA polymerase deficient virus. |
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#17 |
Generation and characterization of BmNPV-based piggyBac baculoviruses for efficient and stable gene transduction in mammalian HEK293 cell line. |
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#18 |
Evaluation of uptake of dsRNA in cell cultures of the insect midgut. |
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#19 |
Microarray-based gene expression profiles in stage-specific larval body markings of the swallowtail butterfly, Papilio xuthus . |
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#20 |
The expression levels of the housekeeping genes in various silkworm, Bombyx mori, strains. |
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#21 |
docPHRP and RARA, two novel family genes induced in response to Bacillus thuringiensis toxins in different Lepidoptera species. |
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#22 |
Cloning, sequencing and classification of midgut aminopeptidases from Ostrinia nubilalis (Hübner). |
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#23 |
A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori. |
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#24 |
Herbivory by Spodoptera littoralis is a trigger for NICTABA lectin in tobacco leaves. |
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#25 |
The midgut transcriptional response underlying the capability of H. armigera larvae to feed on plant organs of different hosts. |
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#26 |
acj6 mutants of the silkworm Bombyx mori cannot discriminate mulberry from beet leaves. |
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#27 |
Deciphering the relationship between gene order and multiple wing patterns in Heliconius numata. L. FREZAL, M. Joron |
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#28 |
The Glanville fritillary butterfly - genetics and genomics |
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upd 11.08.2009
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